Application of digital polymerase chain reaction technology for noninvasive prenatal test
نویسندگان
چکیده
the chromosomal abnormalities, it was reported that Down syndrome (trisomy 21) occurs in 1 in 800 [3,4], Edward syndrome (trisomy 18) in 1 in 6,000 [5], and Patau syndrome (trisomy 13) in 1 in 10,000 fetal subjects [6]. Moreover, birth defects from Down syndrome comprise about 26% of all congenital birth defects [7,8]. As maternal age increases, fetal chromosomal aneuploidy becomes more prevalent [9]. The prevalence of fetal aneuploidy has rapidly increased over the last decade in Korea because of the increasing age of marriage and childbearing (Table 1). Application of digital polymerase chain reaction technology for noninvasive prenatal test
منابع مشابه
Fetal nucleic acids in maternal plasma.
The discovery of cell-free fetal nucleic acids in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis. Over the last few years, a number of approaches have been demonstrated to allow such circulating fetal nucleic acids to be used for the prenatal detection of chromosomal aneuploidies. One such approach involves the enrichment of fetal DNA, such as by size fractio...
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OBJECTIVE This study aims to develop a noninvasive prenatal test on the basis of the analysis of cell-free DNA in maternal blood to detect fetal aneuploidy at chromosomes 13, 18, 21, X, and Y. METHODS A total of 166 samples from pregnant women, including 11 trisomy 21, three trisomy 18, two trisomy 13, two 45,X, and two 47,XXY samples, were analyzed using an informatics-based method. Cell-fre...
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